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Potential biomarkers in the urine of myocardial infarction rats: a metabolomic method and its application.
- Source :
-
Molecular bioSystems [Mol Biosyst] 2011 Mar; Vol. 7 (3), pp. 824-31. Date of Electronic Publication: 2010 Dec 10. - Publication Year :
- 2011
-
Abstract
- A metabolomic method using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to obtain a systematic view of the development and progression of myocardial infarction (MI). By combining with partial least squares discriminant analysis (PLS-DA), 16 biomarkers in rat urine were identified and eight of them were related to the pathway of energy metabolism. Among the regulated pathways, the citric acid cycle related network was acutely perturbed. The metabolomic results not only supplied a systematic view of the development and progression of MI but also provided the theoretical basis for the prevention or treatment of MI. The developed method was also used to analyze the therapeutic effects of a traditional Chinese medicine (TCM) named Shexiang Baoxin Pill (SBP), a widely used anti-MI medicine in clinics. The results showed that SBP administration could provide satisfactory effects on MI through partially regulating the perturbed pathway of energy metabolism.
- Subjects :
- Animals
Biomarkers metabolism
Chromatography, Reverse-Phase
Disease Models, Animal
Drugs, Chinese Herbal administration & dosage
Drugs, Chinese Herbal therapeutic use
Energy Metabolism
Least-Squares Analysis
Male
Mass Spectrometry
Metabolomics
Myocardial Infarction drug therapy
Myocardial Infarction metabolism
Rats
Rats, Sprague-Dawley
Biomarkers urine
Myocardial Infarction urine
Subjects
Details
- Language :
- English
- ISSN :
- 1742-2051
- Volume :
- 7
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular bioSystems
- Publication Type :
- Academic Journal
- Accession number :
- 21152560
- Full Text :
- https://doi.org/10.1039/c0mb00110d