Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.

Authors :: Estrozi LF
Boehringer D
Shan SO
Ban N
Schaffitzel C
Source :: Nature structural & molecular biology [Nat Struct Mol Biol] 2011 Jan; Vol. 18 (1), pp. 88-90. Date of Electronic Publication: 2010 Dec 12.
Publication Year :: 2011
Abstract: We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

Subjects :: Bacterial Proteins metabolism
Cryoelectron Microscopy
Escherichia coli Proteins metabolism
Models, Molecular
Protein Biosynthesis
Protein Structure, Tertiary
Receptors, Cytoplasmic and Nuclear metabolism
Ribosomes chemistry
Signal Recognition Particle metabolism
Bacterial Proteins chemistry
Escherichia coli genetics
Escherichia coli Proteins chemistry
Receptors, Cytoplasmic and Nuclear chemistry
Receptors, Peptide chemistry
Ribosomes ultrastructure
Signal Recognition Particle chemistry

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