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Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Müller glial cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Jan 14; Vol. 404 (2), pp. 678-83. Date of Electronic Publication: 2010 Dec 11. - Publication Year :
- 2011
-
Abstract
- Normal vision depends on the correct function of retinal neurons and glia and it is impaired in the course of diabetic retinopathy. Müller cells, the main glial cells of the retina, suffer morphological and functional alterations during diabetes participating in the pathological retinal dysfunction. Recently, we showed that Müller cells express the pleiotropic protein potassium channel interacting protein 3 (KChIP3), an integral component of the voltage-gated K(+) channels K(V)4. Here, we sought to analyze the role of KChIP3 in the molecular mechanisms underlying hyperglycemia-induced phenotypic changes in the glial elements of the retina. The expression and function of KChIp3 was analyzed in vitro in rat Müller primary cultures grown under control (5.6 mM) or high glucose (25 mM) (diabetic-like) conditions. We show the up-regulation of KChIP3 expression in Müller cell cultures under high glucose conditions and demonstrate a previously unknown interaction between the K(V)4 channel and KChIP3 in Müller cells. We show evidence for the expression of a 4-AP-sensitive transient outward voltage-gated K(+) current and an alteration in the inactivation of the macroscopic outward K(+) currents expressed in high glucose-cultured Müller cells. Our data support the notion that induction of KChIP3 and functional changes of K(V)4 channels in Müller cells could exert a physiological role in the onset of diabetic retinopathy.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cells, Cultured
Diabetic Retinopathy metabolism
Diabetic Retinopathy pathology
Glucose physiology
Hyperglycemia pathology
Kv Channel-Interacting Proteins metabolism
Kv Channel-Interacting Proteins physiology
Neuroglia drug effects
Neuroglia pathology
Rats
Rats, Long-Evans
Retina drug effects
Retina pathology
Shal Potassium Channels physiology
Glucose metabolism
Hyperglycemia metabolism
Kv Channel-Interacting Proteins biosynthesis
Neuroglia metabolism
Repressor Proteins biosynthesis
Retina metabolism
Shal Potassium Channels biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 404
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 21147063
- Full Text :
- https://doi.org/10.1016/j.bbrc.2010.12.041