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Expression of Ezrin, HGF, C-met in pancreatic cancer and non-cancerous pancreatic tissues of rats.
- Source :
-
Hepatobiliary & pancreatic diseases international : HBPD INT [Hepatobiliary Pancreat Dis Int] 2010 Dec; Vol. 9 (6), pp. 639-44. - Publication Year :
- 2010
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Abstract
- Background: Recent studies have confirmed that the expression of Ezrin, hepatocyte growth factor (HGF) and its receptor (C-met) is related to the genesis, progress, invasion and metastasis of some malignant tumors. Researches have also found that the biological function of Ezrin is closely related to HGF/C-met in malignant tumors. However, there is no report on the expression levels of Ezrin, HGF and C-met in rat pancreatic cancer induced by dimethylbenzanthracene (DMBA). This study aimed to detect the expression of Ezrin, HGF and C-met in rat pancreatic cancer and non-cancerous pancreatic tissues, and assess its effect in cancer induction by DMBA.<br />Methods: Ninety Sprague-Dawley rats were divided into 3 groups randomly: 40 in a pancreatic cancer model group (group A), 40 in a trichostatin A (TSA) intervention group (group B), and 10 in a control group (group C). DMBA was directly implanted into the parenchyma of rat pancreas in group A+group B. The rats of group B were treated with 1 ml of TSA saline solution (1 μg/ml) via intraperitoneal injection weekly. The carcinogenesis of rats executed within 3-5 months in groups A and B was observed by macrograph and microscopy. Meanwhile, the rats in group C were executed within 5 months. The EnVisionTM immunohistochemistry for detecting the expression levels of Ezrin, HGF and C-met was used in paraffin-embedded sections of the pancreatic specimens.<br />Results: The incidence of pancreatic cancer in group A was 48.6% and in group B 33.3%. The maximal diameter of tumor mass was significantly larger in group A than that in group B (P<0.05). No pathological changes were observed in the pancreas of group C and other main organs of groups A and B. The positive rates of Ezrin, HGF and C-met were significantly higher in ductal adenocarcinoma than in non-cancerous pancreatic tissues of groups A and B (P<0.01). The positive rates of Ezrin, HGF and C-met were significantly higher in ductal adenocarcinoma of group A than those in non-cancerous pancreatic tissues of group A (P<0.05), but there was no significant difference in group B (P>0.05). The positive rates of Ezrin, HGF and C-met in non-cancerous pancreatic tissues proved mild to severe atypical hyperplasia of the ductal epithelia. The pancreas of group C and 2 cases of fibrosarcoma showed the negative expression of Ezrin, HGF and C-met. There was a trend of consistency in the expression of Ezrin, HGF and C-met in ductal adenocarcinoma (P<0.05 or P<0.01).<br />Conclusions: DMBA directly implanted into the parenchyma of the pancreas can produce a model of pancreatic cancer with a high incidence in a short time. TSA might inhibit the carcinogenesis and growth of pancreatic cancer, and its effects may be related to the inhibition of the expression of Ezrin, HGF and C-met during the process. Ezrin, HGF and C-met may have positive effects on the carcinogenesis of rat pancreas.
- Subjects :
- 9,10-Dimethyl-1,2-benzanthracene toxicity
Adenocarcinoma chemically induced
Adenocarcinoma pathology
Animals
Carcinogens toxicity
Carcinoma, Pancreatic Ductal chemically induced
Carcinoma, Pancreatic Ductal metabolism
Carcinoma, Pancreatic Ductal pathology
Disease Models, Animal
Fibrosarcoma chemically induced
Fibrosarcoma metabolism
Fibrosarcoma pathology
Immunohistochemistry
Pancreas metabolism
Pancreas pathology
Pancreatic Neoplasms chemically induced
Pancreatic Neoplasms pathology
Rats
Rats, Sprague-Dawley
Adenocarcinoma metabolism
Cytoskeletal Proteins metabolism
Hepatocyte Growth Factor metabolism
Pancreatic Neoplasms metabolism
Proto-Oncogene Proteins c-met metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1499-3872
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Hepatobiliary & pancreatic diseases international : HBPD INT
- Publication Type :
- Academic Journal
- Accession number :
- 21134835