Back to Search
Start Over
Expression of multiple membrane-associated phospholipase A1 beta transcript variants and lysophosphatidic acid receptors in Ewing tumor cells.
- Source :
-
Molecular biology reports [Mol Biol Rep] 2011 Oct; Vol. 38 (7), pp. 4619-28. Date of Electronic Publication: 2010 Dec 04. - Publication Year :
- 2011
-
Abstract
- The prognosis for patients with advanced stages of Ewing family tumors (EFT) is very poor. EFT express high levels of phosphatidic acid specific membrane-associated phospholipase A1 beta (lipase I, LIPI). LIPI is a cancer/testis antigen and the high tumor specificity suggests that LIPI might be an attractive target for new diagnostic and/or therapeutic developments. By using reverse transcriptase-polymerase chain reaction (RT-PCR), we observed simultaneous presence of multiple LIPI transcript variants in EFT. We cloned and sequenced these transcript variants from EFT cell lines. Sequence analysis indicated that all transcript variants were derived by alternative splicing. Homology modeling of corresponding protein structures suggested that different transcript variants differ in their regulatory lid domains. In addition, expression of receptors for lysophosphatidic acid (LPA) was analyzed in a panel of EFT cell lines by RT-PCR. We observed that EFT cell lines expressed high levels of LPA receptors. Different LIPI transcript variants present in EFT might be involved in the pathogenesis of EFT by signaling via these LPA receptors.
- Subjects :
- Cell Line, Tumor
Exons genetics
Humans
Lipase genetics
Lipase metabolism
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide genetics
RNA Splice Sites genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Lysophosphatidic Acid metabolism
Structural Homology, Protein
Alternative Splicing genetics
Antigens, Neoplasm genetics
Cell Membrane enzymology
Gene Expression Regulation, Neoplastic
Phospholipases A1 genetics
Receptors, Lysophosphatidic Acid genetics
Sarcoma, Ewing enzymology
Sarcoma, Ewing genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 38
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 21132378
- Full Text :
- https://doi.org/10.1007/s11033-010-0595-z