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DAP10 contributes to CD8(+) T cell-mediated cytotoxic effector mechanisms during Mycobacterium tuberculosis infection.
- Source :
-
Immunobiology [Immunobiology] 2011 May; Vol. 216 (5), pp. 639-47. Date of Electronic Publication: 2010 Sep 24. - Publication Year :
- 2011
-
Abstract
- The activating C-type lectin-like receptor NKG2D, which is expressed by mouse NK cells and activated CD8 T cells, was previously demonstrated to be involved in tumor rejection and as a defense mechanism against viral and bacterial infections. Because CD8 T cells are important for protective immune responses during chronic Mycobacterium tuberculosis (Mtb) infection and represent a promising target for new vaccine strategies to prevent human pulmonary tuberculosis (TB), we studied the immune response in mice deficient for the NKG2D adapter molecule DAP10 during experimental TB. After aerosol infection, DAP10-defcient mice displayed an unimpaired recruitment, activation and development of antigen-specific CD8 T cells. Whereas the frequency of interferon-gamma-producing CD8 T cells from Mtb-infected DAP10-defcient mice was not affected, CD8 T cell-mediated cytotoxicity was significantly reduced in the absence of DAP10. The loss of cytotoxic activity in DAP10-deficient CD8 T cells was associated with an impaired release of cytotoxic granules. Together, our results suggest that during Mtb infection DAP10 is required for maximal cytolytic activity of CD8 T cells.<br /> (Copyright © 2010 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes microbiology
CD8-Positive T-Lymphocytes pathology
Cell Count
Cells, Cultured
Disease Models, Animal
Humans
Interferon-gamma metabolism
Lymphocyte Activation genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Mycobacterium tuberculosis pathogenicity
Receptors, Immunologic genetics
Receptors, Immunologic immunology
Tuberculosis, Pulmonary drug therapy
CD8-Positive T-Lymphocytes metabolism
Cytotoxicity, Immunologic genetics
Mycobacterium tuberculosis immunology
Receptors, Immunologic metabolism
Tuberculosis, Pulmonary immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 216
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 21122940
- Full Text :
- https://doi.org/10.1016/j.imbio.2010.09.010