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Determination of benzo[a]pyrene diol epoxide-DNA adducts in white blood cell DNA from coke-oven workers: the impact of smoking.
- Source :
-
Journal of the National Cancer Institute [J Natl Cancer Inst] 1990 Jun 06; Vol. 82 (11), pp. 927-33. - Publication Year :
- 1990
-
Abstract
- We have undertaken a study among coke-oven workers to test the feasibility of an enzyme-linked immunosorbent assay with anti-trans-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene- DNA antibodies for monitoring occupational exposure to polycyclic aromatic hydrocarbons (PAH). Coke-oven workers are occupationally exposed to relatively high levels of PAH and are at increased risk for lung cancer. Three blood samples were collected from each of the 56 coke-oven workers exposed to PAH and 44 unexposed workers employed in a steel-rolling factory of the same plant. In addition, PAH levels were measured in ambient air by personal sampling, and the excretion of 1-hydroxypyrene in urine was also measured on 3 consecutive working days. All participants were interviewed regarding working conditions, personal hygiene, and smoking habits. The results showed that the coke-oven workers were exposed to substantial concentrations of atmospheric PAH (1-186 micrograms/m3), including benzo[a]pyrene (0.1-7.8 micrograms/m3) and pyrene (0.6-23.6 micrograms/m3). Both benzo[a]pyrene and pyrene were shown to be representative for the whole group of PAH. Forty-seven percent of the coke-oven workers had detectable levels of PAH-DNA adducts in their white blood cells, compared with 30% of the controls. In both groups, smokers had significantly higher levels of PAH-DNA adducts than did nonsmokers. At one site, we found the correlation positive between DNA adducts and the duration of exposure (r = .47, P = .005). Generally, the correlation was not significant between PAH-DNA adducts in blood and the concentration of PAH in the air and 1-hydroxypyrene in urine.
- Subjects :
- Adult
Enzyme-Linked Immunosorbent Assay
Humans
Middle Aged
Mutagens urine
Polycyclic Compounds analysis
Pyrenes metabolism
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide analysis
Air Pollutants, Occupational
Coal
Coke
DNA analysis
Dihydroxydihydrobenzopyrenes analysis
Leukocytes analysis
Smoking adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8874
- Volume :
- 82
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of the National Cancer Institute
- Publication Type :
- Academic Journal
- Accession number :
- 2111410
- Full Text :
- https://doi.org/10.1093/jnci/82.11.927