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Improvement of spinosad production by overexpression of gtt and gdh controlled by promoter PermE* in Saccharopolyspora spinosa SIPI-A2090.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2011 Apr; Vol. 33 (4), pp. 733-9. Date of Electronic Publication: 2010 Nov 25. - Publication Year :
- 2011
-
Abstract
- Spinosyns A and D are novel macrolide secondary metabolites containing two deoxysugars. Each of the sugars is derived from a common intermediate, which is biosynthesized sequentially by NDP-glucose synthase and glucose dehydratase. Systematical duplication of gtt and gdh was carried out by genetic engineering in Saccharopolyspora spinosa. Introduction of additional gtt and gdh genes under the control of PermE* promoter showed significant increase on their transcript levels. The production of spinosad was increased by over threefold to reach 800 μg/ml in the recombinant strain SIPI-M2093.
- Subjects :
- Biotechnology
Drug Combinations
Gene Duplication
Molecular Structure
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Bacterial genetics
Glucose 1-Dehydrogenase genetics
Glucose 1-Dehydrogenase metabolism
Hydro-Lyases genetics
Hydro-Lyases metabolism
Macrolides metabolism
Promoter Regions, Genetic
Saccharopolyspora genetics
Saccharopolyspora metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 33
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 21107647
- Full Text :
- https://doi.org/10.1007/s10529-010-0481-8