Tyrosine phosphorylation enrichment and subsequent analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS.

Authors :: Condina MR
Klingler-Hoffmann M
Hoffmann P
Source :: Current protocols in protein science [Curr Protoc Protein Sci] 2010 Nov; Vol. Chapter 13, pp. Unit13.11.
Publication Year :: 2010
Abstract: This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods.<br /> (© 2010 by John Wiley & Sons, Inc.)

Subjects :: Antibodies, Phospho-Specific
Chromatography, Liquid methods
Peptide Mapping methods
Phosphorylation
Phosphotyrosine chemistry
Protein Processing, Post-Translational
Proteins analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Tyrosine chemistry
Chemistry Techniques, Analytical
Phosphotyrosine analysis
Proteins chemistry

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