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Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926.
- Source :
-
Radiation and environmental biophysics [Radiat Environ Biophys] 2011 Mar; Vol. 50 (1), pp. 155-66. Date of Electronic Publication: 2010 Nov 23. - Publication Year :
- 2011
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Abstract
- High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100-500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in the cytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24 h after irradiation at two different dose rates (Co-60 gamma ray total dose 200 mGy; 20 mGy/min and 190 mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than ±1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response.<br /> (© Springer-Verlag 2010)
- Subjects :
- Apoptosis radiation effects
Cell Proliferation radiation effects
Cytosol metabolism
Cytosol radiation effects
Dose-Response Relationship, Drug
Endothelial Cells cytology
Gene Expression Profiling
Humans
Proteomics
Radiography
Time Factors
Endothelial Cells diagnostic imaging
Endothelial Cells metabolism
Proteome metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2099
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Radiation and environmental biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 21104263
- Full Text :
- https://doi.org/10.1007/s00411-010-0342-9