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Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study.
- Source :
-
Molecular biology reports [Mol Biol Rep] 2011 Apr; Vol. 38 (4), pp. 2611-7. Date of Electronic Publication: 2010 Nov 23. - Publication Year :
- 2011
-
Abstract
- Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan. Linkage mapping placed the IRS4 gene at position 82 cM on the current USDA-USMARC linkage map of porcine chromosome X. Association analyses were performed on 555 animals of 12th-15th generation of the Meishan × Large White cross and showed that both SNPs were highly significantly associated with backfat depth (P=0.0005) and that the SNP FN424076:g1829T<C was also associated with loin depth (P=0.017). The Meishan alleles increased back fat depth and decreased loin depth. IRS4 can be considered a positional candidate gene for at least some of the QTL located at the centromeric region of porcine chromosome X.
- Subjects :
- Animals
Base Sequence
Body Weights and Measures
Chromosome Mapping
Cloning, Molecular
DNA Primers genetics
Genome-Wide Association Study
Linear Models
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology
Insulin Receptor Substrate Proteins genetics
Phenotype
Polymorphism, Single Nucleotide genetics
Swine genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 21104145
- Full Text :
- https://doi.org/10.1007/s11033-010-0402-x