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Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p.
- Source :
-
Journal of cell science [J Cell Sci] 2010 Dec 15; Vol. 123 (Pt 24), pp. 4374-81. Date of Electronic Publication: 2010 Nov 23. - Publication Year :
- 2010
-
Abstract
- Regulated gene expression makes an important contribution to cell cycle control mechanisms. In fission yeast, a group of genes is coordinately expressed during a late stage of the cell cycle (M phase and cytokinesis) that is controlled by common cis-acting promoter motifs named pombe cell cycle boxes (PCBs), which are bound by a trans-acting transcription factor complex, PCB binding factor (PBF). PBF contains at least three transcription factors, a MADS box protein Mbx1p and two forkhead transcription factors, Sep1p and Fkh2p. Here we show that the fission yeast Cdc14p-like phosphatase Clp1p (Flp1p) controls M-G1 specific gene expression through PBF. Clp1p binds in vivo both to Mbx1p, a MADS box-like transcription factor, and to the promoters of genes transcribed at this cell cycle time. Because Clp1p dephosphorylates Mbx1p in vitro, and is required for Mbx1p cell cycle-specific dephosphorylation in vivo, our observations suggest that Clp1p controls cell cycle-specific gene expression through binding to and dephosphorylating Mbx1p.
- Subjects :
- G1 Phase genetics
Genes, Fungal genetics
Mitosis genetics
Models, Genetic
Phosphorylation
Promoter Regions, Genetic genetics
Protein Binding
Schizosaccharomyces enzymology
Schizosaccharomyces pombe Proteins genetics
Cell Cycle genetics
Cell Cycle Proteins metabolism
Gene Expression Regulation, Fungal
Phosphoprotein Phosphatases metabolism
Protein Tyrosine Phosphatases metabolism
Schizosaccharomyces cytology
Schizosaccharomyces genetics
Schizosaccharomyces pombe Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9137
- Volume :
- 123
- Issue :
- Pt 24
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 21098641
- Full Text :
- https://doi.org/10.1242/jcs.073056