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African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa.
- Source :
-
Journal of bacteriology [J Bacteriol] 2011 Feb; Vol. 193 (3), pp. 670-8. Date of Electronic Publication: 2010 Nov 19. - Publication Year :
- 2011
-
Abstract
- We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.
- Subjects :
- Africa, Eastern epidemiology
Animals
Bacterial Typing Techniques
Cattle
Cluster Analysis
DNA Fingerprinting
DNA Transposable Elements
DNA, Bacterial chemistry
DNA, Bacterial genetics
Gene Dosage
Genotype
Molecular Sequence Data
Mycobacterium bovis genetics
Sequence Analysis, DNA
Sequence Deletion
Mycobacterium bovis classification
Mycobacterium bovis isolation & purification
Tuberculosis, Bovine epidemiology
Tuberculosis, Bovine microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 193
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 21097608
- Full Text :
- https://doi.org/10.1128/JB.00750-10