Back to Search
Start Over
[Effects of AMPK on the transcriptional activity of FOXO1 and ubiquitin ligase MuRF1 expression in rat cardiomyocytes].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2010 Nov; Vol. 30 (11), pp. 2419-22. - Publication Year :
- 2010
-
Abstract
- Objective: To investigate the effects of AICAR on the activity of transcription factor FOXO1 and expression of ubiquitin ligase MuRF1 in rat cardiomyocytes, and explore the possible role of AMP-activated protein kinase (AMPK) in proteolysis pathways.<br />Methods: In vitro cultured neonatal rat cardiac myocytes were treated with AICAR, and Western blotting was used to detect the phosphorylation of FOXO1 and expression of MuRF1 in the cells.<br />Results: AICAR activated AMPK in rat cardiac myocytes. Activated AMPK significantly inhibited the phosphorylation of FOXO1 and increased MuRF1 protein expression.<br />Conclusion: AMPK may regulate proteolysis by activating FOXO1 transcription factor and up-regulating MuRF1 expression.
- Subjects :
- Aminoimidazole Carboxamide analogs & derivatives
Aminoimidazole Carboxamide pharmacology
Animals
Cells, Cultured
Rats
Rats, Sprague-Dawley
Ribonucleotides pharmacology
Tripartite Motif Proteins
AMP-Activated Protein Kinases metabolism
Forkhead Transcription Factors metabolism
Muscle Proteins metabolism
Myocytes, Cardiac metabolism
Nerve Tissue Proteins metabolism
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 30
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 21097394