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Experimental priapism is associated with increased oxidative stress and activation of protein degradation pathways in corporal tissue.
- Source :
-
International journal of impotence research [Int J Impot Res] 2010 Nov-Dec; Vol. 22 (6), pp. 363-73. Date of Electronic Publication: 2010 Nov 18. - Publication Year :
- 2010
-
Abstract
- Priapism is a debilitating disease for which there is at present no clinically accepted pharmacological intervention. It has been estimated that priapism lasting more than 24 h in patients is associated with a 44-90% rate of ED. In this investigation, we determined in two animal models of priapism (opiorphin-induced priapism in the rat and priapism in a mouse model of sickle cell disease) if there is evidence for an increase in markers of oxidative stress in corporal tissue. In both animal models, we demonstrate that priapism results in increased levels of lipid peroxidation, glutathione S-transferase activity and oxidatively damaged proteins in corporal tissue. Using western blot analysis, we demonstrated there is upregulation of the ubiquitination ligase proteins, Nedd-4 and Mdm-2, and the lysosomal autophage protein, LC3. The antiapoptotic protein, Bcl-2, was also upregulated. Overall, we demonstrate that priapism is associated with increased oxidative stress in corporal tissue and the activation of protein degradation pathways. As oxidative stress is known to mediate the development of ED resulting from several etiologies (for example, ED resulting from diabetes and aging), we suggest that damage to erectile tissue resulting from priapism might be prevented by treatments targeting oxidative stress.
- Subjects :
- Anemia, Sickle Cell complications
Animals
Disease Models, Animal
Glutathione Transferase metabolism
Lipid Peroxidation
Male
Mice
Mice, Transgenic
Oligopeptides administration & dosage
Oxidation-Reduction
Penis drug effects
Priapism etiology
Proto-Oncogene Proteins c-bcl-2 analysis
Rats
Rats, Sprague-Dawley
Salivary Proteins and Peptides administration & dosage
Ubiquitin-Protein Ligases analysis
Ubiquitination
Oxidative Stress physiology
Penis metabolism
Priapism metabolism
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5489
- Volume :
- 22
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of impotence research
- Publication Type :
- Academic Journal
- Accession number :
- 21085184
- Full Text :
- https://doi.org/10.1038/ijir.2010.27