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[Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy].
- Source :
-
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics [Zhongguo Dang Dai Er Ke Za Zhi] 2010 Nov; Vol. 12 (11), pp. 908-11. - Publication Year :
- 2010
-
Abstract
- Objective: To study the relationship of activated astrocytes and multidrug resistance gene (MDR) expression in rats with epilepsy.<br />Methods: Astrocytes of neonatal Sprague-Dawley rats were separated and cultured. The cultured cells of passage 3 were activated by TNF-α for 2, 24 or 48 hrs. The culture media of cells with different degrees of proliferation were infused to the lateral cerebral ventricle of rats with epilepsy. The expression of MDR in the brain tissue was ascertained by PCR, immunocytochemistry and Western blot.<br />Results: After 2 hrs of TNF-α stimulation, astrocytes began to proliferate, and reached a peak at 24 hrs. The expression of MDR in the brain tissue increased after infusion of culture medium of proliferated astrocytes in the TNF stimulation group compared with that in the control group without TNF stimulation. The level of MDR expression in the TNF stimulation group was positively correlated with the degrees of cell proliferation.<br />Conclusions: Proliferation of astrocytes can increase the expression of MDR in rats with epilepsy and is probably involved in the development of refractory epilepsy.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 1 analysis
Animals
Cell Proliferation
Drug Resistance, Multiple genetics
Female
Male
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha pharmacology
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Astrocytes physiology
Epilepsy metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-8830
- Volume :
- 12
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 21083989