Disturbed apolipoprotein A-I-containing lipoproteins in fish-eye disease are improved by the lecithin:cholesterol acyltransferase produced by gene-transduced adipocytes in vitro.

Authors :: Asada S
Kuroda M
Aoyagi Y
Bujo H
Tanaka S
Konno S
Tanio M
Ishii I
Aso M
Saito Y
Source :: Molecular genetics and metabolism [Mol Genet Metab] 2011 Feb; Vol. 102 (2), pp. 229-31. Date of Electronic Publication: 2010 Oct 20.
Publication Year :: 2011
Abstract: We report the in vitro efficacy of recombinant LCAT produced by lcat gene-transduced proliferative adipocytes (ccdPA/lcat), which has been developed for enzyme replacement therapy. ApoA-I-specific immunodetection in combination with 1D and 2D gel electrophoreses showed that the disturbed high-density lipoprotein subpopulation profile was clearly ameliorated by the in vitro incubation with ccdPA/lcat-derived recombinant LCAT. Thus, these results using ccdPA/lcat strongly suggest the cell implantation could contribute the enzyme replacement for the patients with LCAT deficiency.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)

Subjects :: Cells, Cultured
Gene Transfer Techniques
Genetic Vectors genetics
Humans
In Vitro Techniques
Male
Phosphatidylcholine-Sterol O-Acyltransferase blood
Phosphatidylcholine-Sterol O-Acyltransferase genetics
Adipocytes enzymology
Apolipoprotein A-I genetics
Apolipoprotein A-I metabolism
Lecithin Cholesterol Acyltransferase Deficiency enzymology
Lecithin Cholesterol Acyltransferase Deficiency genetics
Phosphatidylcholine-Sterol O-Acyltransferase metabolism

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