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Induction of cytokine formation by human intestinal bacteria in gut epithelial cell lines.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2011 Jan; Vol. 110 (1), pp. 353-63. Date of Electronic Publication: 2010 Nov 10. - Publication Year :
- 2011
-
Abstract
- Aims: To investigate the effects of human gut micro-organisms on cytokine production by human intestinal cell lines.<br />Methods and Results: Quantitative real-time PCR assays were developed to measure the production of pro-inflammatory (IL-1α, IL-6, IL-18 and TNFα) and anti-inflammatory (TGF-β1, TGF-β2, TGF-β3, IL-4 and IL-10) cytokines in HT-29 and Caco-2 cell lines. They were co-cultured with a range of mucosal bacteria isolated from ulcerative colitis patients, together with lactobacilli and bifidobacteria obtained from healthy people. HT-29 cells were also co-cultured with Campylobacter jejuni, enterotoxigenic Escherichia coli (ETEC), enteropathogenic E. coli and Salmonella typhimurium. The majority of commensal bacteria tested suppressed the expression of anti-inflammatory cytokine mRNA, increased IL-18, reduced IL-1α, and with the exception of nonpathogenic E. coli, reduced TNF-α. All overtly pathogenic species increased both pro-inflammatory and anti-inflammatory cytokine mRNA.<br />Conclusion: Commensal and pathogenic species induced fundamentally different cytokine responses in human intestinal epithelial cell lines.<br />Significance and Impact of the Study: Interactions between commensal bacteria tested in this study and the innate immune system were shown to be anti-inflammatory in nature, in contrast to the pathogenic organisms investigated. These data contribute towards our understanding of how potential probiotic species can be used to suppress the pro-inflammatory response in inflammatory bowel disease.<br /> (© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology.)
- Subjects :
- Bifidobacterium physiology
Caco-2 Cells
Campylobacter jejuni physiology
Coculture Techniques
Colitis, Ulcerative microbiology
Cytokines genetics
Epithelial Cells immunology
Epithelial Cells microbiology
Escherichia coli physiology
HT29 Cells
Humans
Interleukins biosynthesis
Interleukins genetics
Lactobacillus physiology
Probiotics
Salmonella typhimurium physiology
Transforming Growth Factor beta biosynthesis
Transforming Growth Factor beta genetics
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha genetics
Bacterial Physiological Phenomena
Cytokines biosynthesis
Intestinal Mucosa immunology
Intestinal Mucosa microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 110
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21070518
- Full Text :
- https://doi.org/10.1111/j.1365-2672.2010.04889.x