Back to Search
Start Over
Characterization of antibodies specific for hemagglutinin and neuraminidase proteins of the 1918 and 2009 pandemic H1N1 viruses.
- Source :
-
Vaccine [Vaccine] 2010 Dec 16; Vol. 29 (2), pp. 183-90. Date of Electronic Publication: 2010 Nov 04. - Publication Year :
- 2010
-
Abstract
- Serologic studies have detected protective immunity against 2009 pandemic H1N1 influenza virus (H1N1-2009) in some people. However, further study of preexisting immunity has been complicated by the complexity of the human immunological background. Here, we immunized mice with HA- and NA-encoding plasmids. The cross-neutralizing activity of the anti-HA antisera and the effect of the anti-NA antisera on viral infectivity were evaluated using H1N1-1918- and 2009-pseudotyped particles (pps) and an H1N1-2009 isolate. Antibodies to H1N1-2009 HA (09HA) neutralized pps harboring 09HA or H1N1-1918 HA (18HA); similarly, antibodies to 18HA neutralized pps harboring 18HA or 09HA. Antibodies to 09HA and 18HA also neutralized the H1N1-2009 virus with high efficiency. Antibodies to H1N1-1918 NA (18NA) or H1N1-2009 NA (09NA) both enhanced the infectivity of pps harboring 09NA and 18NA. Although anti-09NA and -18NA antibodies significantly reduced cytopathic effects in multiple-cycle infection assays, conversely, these antibodies enhanced the infectivity of H1N1-2009 in single-cycle infection assays. Our study demonstrates the existence of cross-protection between antibodies against these two antigenically related virus strains and shows that anti-NA antibodies have a dual effect that requires reexamination of their role in human immunity.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Blocking immunology
Antibodies, Neutralizing blood
Antibodies, Neutralizing immunology
Cell Line
Cross Protection
Cross Reactions
Cytopathogenic Effect, Viral
Dogs
Female
Genetic Vectors
Hemagglutinins, Viral genetics
Humans
Influenza Vaccines administration & dosage
Influenza Vaccines immunology
Mice
Mice, Inbred BALB C
Neuraminidase genetics
Neutralization Tests
Plasmids
Vaccines, DNA administration & dosage
Vaccines, DNA immunology
Viral Proteins genetics
Antibodies, Viral blood
Antibodies, Viral immunology
Hemagglutinins, Viral immunology
Influenza A Virus, H1N1 Subtype immunology
Neuraminidase immunology
Viral Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 21055499
- Full Text :
- https://doi.org/10.1016/j.vaccine.2010.10.059