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DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa.
- Source :
-
Journal of bacteriology [J Bacteriol] 1990 Feb; Vol. 172 (2), pp. 853-66. - Publication Year :
- 1990
-
Abstract
- Two pairs of related but easily distinguishable genes for the two subunits of anthranilate synthase have been identified in Pseudomonas aeruginosa. These were cloned, sequenced, inactivated in vitro by insertion of an antibiotic resistance cassette, and returned to the P. aeruginosa chromosome, replacing the wild-type gene. Gene replacement implicated only one of the pairs in tryptophan biosynthesis. This report describes the cloning and sequencing of the tryptophan-related gene pair, designated trpE and trpG, and presents experiments implicating their gene products in tryptophan production. DNA sequence analysis as well as growth and enzyme assays of insertionally inactivated strains indicated that trpG is the first gene in a three-gene operon that also includes trpD and trpC. Complementation of Trp auxotrophs by R-prime plasmids (T. Shinomiya, S. Shiga, and M. Kageyama, Mol. Gen. Genet., 189:382-389, 1983) has shown that a large cluster of pyocin R2 genes is flanked at one end by trpE and the other end by trpDC; the physical map that was obtained shows the distance between trpE and trpDC to be about 25 kilobases. Our restriction map of the trpE and trpGDC regions agrees with data presented by Shinomiya et al.
- Subjects :
- Amino Acid Sequence
Base Sequence
Blotting, Southern
Conjugation, Genetic
DNA, Bacterial isolation & purification
Escherichia coli genetics
Genotype
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
Operon
Phenotype
Plasmids
Pseudomonas aeruginosa enzymology
Pseudomonas aeruginosa growth & development
Restriction Mapping
Sequence Homology, Nucleic Acid
Transformation, Genetic
Anthranilate Synthase genetics
DNA, Bacterial genetics
Genes, Bacterial
Pseudomonas aeruginosa genetics
Tryptophan biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 172
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 2105306
- Full Text :
- https://doi.org/10.1128/jb.172.2.853-866.1990