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Comparison of rhesus and cynomolgus macaques in a Streptococcus pyogenes infection model for vaccine evaluation.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2011 Jan; Vol. 50 (1), pp. 39-47. Date of Electronic Publication: 2010 Oct 28. - Publication Year :
- 2011
-
Abstract
- Animal models predictive of human disease are generally difficult to establish and reproduce. In the case of the Group A Streptococcus (GAS) bacterium, which is predominantly a human pathogen, virulence assessment in animal models is problematic. We compared a monkey colonization and pharyngitis model of infection in two macaque species to determine the optimal model for vaccine candidate evaluation. Rhesus and cynomolgus macaques were intranasally infected with a streptomycin resistant (Str(r)) GAS strain. Monkeys were monitored for body weight and temperature changes, throat swabs and sera were collected, and clinical observations were noted throughout the study. Both species exhibited oropharyngeal colonization by GAS, with rhesus macaques demonstrating a more sustained colonization through day 28 post-challenge. Veterinary observations revealed no significant differences between GAS-infected rhesus and cynomolgus macaques. Mock-infected monkeys did not exhibit clinical symptoms or GAS colonization throughout the study. ELISA results demonstrated that both rhesus and cynomolgus macaques developed anti-streptolysin-O antibody titers, with cynomolgus generating higher titers. Sera from infected monkeys produced opsonophagocytic killing and bound to the bacterium in an immunofluorescence assay. Both rhesus and cynomolgus macaques can be used for colonization studies with this GAS M3 strain, yet only mild clinical signs of pharyngitis and tonsillitis were observed.<br /> (Copyright © 2010. Published by Elsevier India Pvt Ltd.)
- Subjects :
- Animals
Antibodies, Bacterial blood
Bacterial Vaccines
Drug Resistance, Bacterial genetics
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Male
Phagocytosis
Streptococcal Infections microbiology
Macaca fascicularis immunology
Macaca mulatta immunology
Models, Animal
Streptococcal Infections immunology
Streptococcus pyogenes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 21035535
- Full Text :
- https://doi.org/10.1016/j.micpath.2010.10.004