Two-step stacking in capillary zone electrophoresis featuring sweeping and micelle to solvent stacking: I. Organic cations.

Authors :: Quirino JP
Source :: Journal of chromatography. A [J Chromatogr A] 2010 Dec 03; Vol. 1217 (49), pp. 7776-80. Date of Electronic Publication: 2010 Oct 27.
Publication Year :: 2010
Abstract: Two-step stacking of organic cations by sweeping and micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE) is presented. The simple procedure involves hydrodynamic injection of a micellar sodium dodecyl sulfate solution before the sample that is prepared without the micelles. The micelles sweep and transport the cations to the boundary zone between the sample and CZE buffer. The presence of organic solvent in the CZE buffer induces the second stacking step of MSS. The LODs obtained for the four beta blocker and two tricyclic antidepressant test drugs were 20-50 times better compared to typical injection.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)

Subjects :: Adrenergic beta-Antagonists analysis
Adrenergic beta-Antagonists chemistry
Antidepressive Agents, Tricyclic analysis
Antidepressive Agents, Tricyclic chemistry
Cations analysis
Linear Models
Organic Chemicals analysis
Reproducibility of Results
Sensitivity and Specificity
Sodium Dodecyl Sulfate
Cations chemistry
Electrophoresis, Capillary methods
Micelles
Organic Chemicals chemistry

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