Interaction of an antidepressant buzepide methiodide with DNA immobilized on the glassy carbon electrode.

Authors :: Kalanur SS
Seetharamappa J
Prashanth SN
Source :: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2011 Feb 01; Vol. 82 (2), pp. 438-42. Date of Electronic Publication: 2010 Sep 29.
Publication Year :: 2011
Abstract: In the present study, a DNA-biosensor was prepared using immobilization technique to investigate the interaction between an antidepressant, buzepide methiodide (BZP) and calf thymus DNA. BZP showed a quasireversible peak in Britton-Robinson (BR) buffer of pH 5 at bare glassy carbon electrode (GCE). At DNA modified GCE, the peak potential of BZP was observed to be shifted towards positive potential revealing intercalative mode of binding. The binding constant and stoichiometry between DNA and BZP are calculated to be 1.908×10(5)M(-1) and 0.982, respectively. The spectroscopic techniques viz., spectrofluorescence and UV-vis absorption have also been employed to understand the interaction between BZP and DNA. The results serve as a reference for the interaction of BZP with DNA base pairs in the natural environment of living cells.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)

Subjects :: Antidepressive Agents administration & dosage
Azepines administration & dosage
Biosensing Techniques
Buffers
DNA, Single-Stranded chemistry
Electrochemistry methods
Electrodes
Glass
Hydrogen-Ion Concentration
Kinetics
Spectrometry, Fluorescence methods
Spectrophotometry, Ultraviolet methods
Antidepressive Agents pharmacology
Azepines pharmacology
Carbon chemistry
DNA chemistry

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