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Fank1 interacts with Jab1 and regulates cell apoptosis via the AP-1 pathway.
- Source :
-
Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2011 Jun; Vol. 68 (12), pp. 2129-39. Date of Electronic Publication: 2010 Oct 27. - Publication Year :
- 2011
-
Abstract
- Regulation of apoptosis at various stages of differentiation plays an important role in spermatogenesis. Therefore, the identification and characterisation of highly expressed genes in the testis that are involved in apoptosis is of great value to delineate the mechanism of spermatogenesis. Here, we reported that Fank1, a novel gene highly expressed in testis, functioned as an anti-apoptotic protein that activated the activator protein 1 (AP-1) pathway. We found that Jab1 (Jun activation domain-binding protein 1), a co-activator of AP-1, specifically interacted with Fank1. Reporter analyses showed that Fank1 activated AP-1 pathway in a Jab1-dependent manner. Fank1 overexpression also increased the expression and activation of endogenous c-Jun. Further study showed that Fank1 inhibited cell apoptosis by upregulating and activating endogenous c-Jun and its downstream target, Bcl-3. This process was shown to be Jab1 dependent. Taken together, our results indicated that by interacting with Jab1, Fank1 could suppress cell apoptosis by activating the AP-1-induced anti-apoptotic pathway.
- Subjects :
- Animals
B-Cell Lymphoma 3 Protein
COP9 Signalosome Complex
Cell Line
DNA-Binding Proteins physiology
Humans
Intracellular Signaling Peptides and Proteins physiology
Peptide Hydrolases physiology
Proto-Oncogene Proteins metabolism
Proto-Oncogene Proteins c-jun metabolism
Transcription Factor AP-1 metabolism
Transcription Factors physiology
Transfection
Apoptosis
DNA-Binding Proteins metabolism
Intracellular Signaling Peptides and Proteins metabolism
Peptide Hydrolases metabolism
Signal Transduction
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1420-9071
- Volume :
- 68
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cellular and molecular life sciences : CMLS
- Publication Type :
- Academic Journal
- Accession number :
- 20978819
- Full Text :
- https://doi.org/10.1007/s00018-010-0559-4