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The effect of Streptococcus pneumoniae pneumolysin on human respiratory epithelium in vitro.
- Source :
-
Microbial pathogenesis [Microb Pathog] 1990 Oct; Vol. 9 (4), pp. 275-84. - Publication Year :
- 1990
-
Abstract
- Streptococcus pneumoniae culture filtrates and pneumolysin both slow human ciliary beating and damage respiratory epithelium in vitro. A polyclonal pneumolysin antibody bound to sepharose beads removed pneumolysin from culture filtrates and showed that pneumolysin alone was responsible for the effects on epithelium. In a 48-h organ culture pneumolysin caused ciliary slowing and epithelial disruption in a dose-dependent manner down to 5 ng/ml. Comparison of the ciliary slowing activity and pneumolysin concentration in filtrates in a continuous broth culture showed a maximal effect at 16 h (pneumolysin 7.5 micrograms/ml). Later the activity decreased while the pneumolysin concentration increased (8.8 micrograms/ml). This loss of activity was prevented by neutralisation of the acid pH of the culture medium. Eight different culture filtrates produced significant (P less than 0.05) ciliary slowing which correlated (r = 0.95) with simultaneously measured haemolytic (pneumolysin) activity. Substitution of tryptophan (position 433) by phenylalanine reduced the haemolytic and ciliary slowing activity of pneumolysin, but did not affect its ability to activate complement. There was no correlation between the ciliary slowing produced by the culture filtrate and that produced by the autolysate of a particular strain, nor between ciliary slowing and the extent of autolysis or the serotype of the strain.
- Subjects :
- Antibodies, Bacterial biosynthesis
Bacterial Proteins
Cell Movement drug effects
Culture Media pharmacology
Dose-Response Relationship, Drug
Epithelium drug effects
Humans
Mutagenesis, Site-Directed
Organ Culture Techniques
Recombinant Proteins pharmacology
Streptococcus pneumoniae metabolism
Streptolysins biosynthesis
Streptolysins immunology
Hemolysin Proteins metabolism
Nasal Mucosa drug effects
Streptococcus pneumoniae pathogenicity
Streptolysins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0882-4010
- Volume :
- 9
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 2097494
- Full Text :
- https://doi.org/10.1016/0882-4010(90)90016-j