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C-terminal armadillo repeats are essential and sufficient for association of the plant U-box armadillo E3 ubiquitin ligase SAUL1 with the plasma membrane.
- Source :
-
Journal of experimental botany [J Exp Bot] 2011 Jan; Vol. 62 (2), pp. 775-85. Date of Electronic Publication: 2010 Oct 18. - Publication Year :
- 2011
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Abstract
- Ubiquitination plays important roles in plant growth and development. Whereas ubiquitin-dependent protein degradation and modulation in the cytoplasm and nucleus are well established in plants, ubiquitination events mediated by E3 ubiquitin ligases at the plasma membrane are largely unknown. Here, it is demonstrated that the suppressor of premature senescence and cell death SENESCENCE-ASSOCIATED UBIQUITIN LIGASE 1 (SAUL1), a plant U-box armadillo repeat (PUB-ARM) E3 ubiquitin ligase, localizes at the plasma membrane. Among the members of the PUB-ARM protein family, this localization is unique to SAUL1 and its two closest homologues. A novel armadillo repeat domain was identified at the SAUL1 C-terminus that directs specific association with the plasma membrane and is crucial for SAUL1 function in vivo. The data suggest that a small subgroup of PUB-ARM proteins including SAUL1 have functions at the plasma membrane probably by modifying target proteins by ubiquitination.
- Subjects :
- Amino Acid Motifs
Arabidopsis chemistry
Arabidopsis genetics
Arabidopsis Proteins genetics
Cell Membrane chemistry
Cell Membrane genetics
Protein Binding
Protein Transport
Ubiquitin-Protein Ligases genetics
Ubiquitination
Arabidopsis enzymology
Arabidopsis Proteins chemistry
Arabidopsis Proteins metabolism
Cell Membrane enzymology
Ubiquitin-Protein Ligases chemistry
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2431
- Volume :
- 62
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 20956359
- Full Text :
- https://doi.org/10.1093/jxb/erq313