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[Isolation and identification of a methanogen from the high temperature oil reservoir water].
- Source :
-
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2010 Jul; Vol. 26 (7), pp. 1009-13. - Publication Year :
- 2010
-
Abstract
- To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60 degrees C, pH 7.0-7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364).
- Subjects :
- Hot Temperature
Methanobacteriaceae genetics
Methanobacteriaceae metabolism
Methanol metabolism
Methanomicrobiaceae genetics
Methanomicrobiaceae isolation & purification
Phylogeny
RNA, Ribosomal, 16S genetics
Water Microbiology
Methanobacteriaceae classification
Methanobacteriaceae isolation & purification
Petroleum microbiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-3061
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 20954404