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Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.
- Source :
-
Clinical biochemistry [Clin Biochem] 2011 Feb; Vol. 44 (2-3), pp. 257-9. Date of Electronic Publication: 2010 Oct 13. - Publication Year :
- 2011
-
Abstract
- Objectives: To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays.<br />Design and Methods: Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli).<br />Results: The SES assay measured 1.2- to 7.2-fold (2.1±0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference.<br />Conclusions: The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.<br /> (Copyright © 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-2933
- Volume :
- 44
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 20950595
- Full Text :
- https://doi.org/10.1016/j.clinbiochem.2010.09.030