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Evaluation of an in vitro assay aimed at measuring protective antibodies against sporozoites.
- Source :
-
Bulletin of the World Health Organization [Bull World Health Organ] 1990; Vol. 68 Suppl, pp. 52-9. - Publication Year :
- 1990
-
Abstract
- We have evaluated the in vitro biological activities of antibodies directed against sporozoites and compared them with their capacity to protect against challenge with both human and rodent malaria. The anti-Plasmodium falciparum antibodies evaluated with the test included monoclonal antibodies (MAbs) NFS1 and NFS2 as well as polyclonal antibodies contained in human hyperimmune sera directed against sporozoites of P. falciparum. The inhibitory effect of these antibodies was dependent on their concentration. However, total inhibition was not observed except occasionally with highly concentrated MAbs (10-100 micrograms/ml). Strong but also incomplete inhibition was observed with sera from humans living in hyperendemic areas. In the P. yoelii rodent system, we tested sera from mice immunized with subunit vaccines. None of these mice were protected in vivo against challenge with 40-200 sporozoites. In vitro only a sub-total inhibition was achieved (maximum 91% at 1:10 serum dilution). In contrast, we tested sera from mice that received NYS1, an IgG3 MAb, in passive transfer and were protected against challenge with 5000 sporozoites. At 1:10 dilution, 100% inhibition was achieved in vitro while IFA titres from these mice were similar to those of vaccinated mice. These data show a close correlation between in vivo and in vitro findings and thus suggest that the inhibition of liver-stage development assay (ILSDA) appears appropriate to evaluate the potential of antibodies.
- Subjects :
- Adult
Aged
Animals
Antibodies, Monoclonal immunology
Fluorescent Antibody Technique
Humans
Immune Sera immunology
Immunization, Passive
In Vitro Techniques
Liver cytology
Mice
Mice, Inbred BALB C
Middle Aged
Antibodies, Protozoan isolation & purification
Malaria prevention & control
Plasmodium falciparum immunology
Plasmodium yoelii immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0042-9686
- Volume :
- 68 Suppl
- Database :
- MEDLINE
- Journal :
- Bulletin of the World Health Organization
- Publication Type :
- Academic Journal
- Accession number :
- 2094592