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Expression of bacterial virulence factors and cytokines during in vitro macrophage infection by enteroinvasive Escherichia coli and Shigella flexneri: a comparative study.
- Source :
-
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2010 Sep; Vol. 105 (6), pp. 786-91. - Publication Year :
- 2010
-
Abstract
- Enteroinvasive Escherichia coli (EIEC) and Shigella spp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigella share many genetic and biochemical similarities, the illness caused by Shigella is more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneri with cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed that S. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri.
- Subjects :
- Cell Death
Enzyme-Linked Immunospot Assay
Escherichia coli genetics
Gene Expression Regulation, Bacterial genetics
Genes, Bacterial
Humans
Reverse Transcriptase Polymerase Chain Reaction
Shigella flexneri genetics
Virulence Factors genetics
Cytokines analysis
Escherichia coli pathogenicity
Gene Expression Regulation, Bacterial immunology
Macrophages microbiology
Shigella flexneri pathogenicity
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1678-8060
- Volume :
- 105
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Memorias do Instituto Oswaldo Cruz
- Publication Type :
- Academic Journal
- Accession number :
- 20944993
- Full Text :
- https://doi.org/10.1590/s0074-02762010000600009