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Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture.
- Source :
-
Journal of plant research [J Plant Res] 2011 May; Vol. 124 (3), pp. 395-403. Date of Electronic Publication: 2010 Oct 13. - Publication Year :
- 2011
-
Abstract
- Sucrose plays an important role in several cellular processes since it is a general source of metabolic energy, serves as a precursor for starch and cellulose synthesis, and is a metabolic starting point for carboxylate- and amino acid synthesis. While plant vacuole is the main cellular storage pool, where sucrose accumulates to high concentrations, only a small number of vacuolar sugar transporters have been identified and characterized to date. We initially identified a vacuolar sucrose transporter (NtSUT4) from tobacco BY-2 cells and established transgenic tobacco BY-2 cell lines that overexpress NtSUT4-GFP (BY-SUTG cells). Using a model system for synchronous cell elongation in miniprotoplasts (evacuolated cells) prepared from tobacco BY-2 cells, we found that NtSUT4-GFP overexpression inhibited cell growth towards the cell major axis. Moreover, under the same conditions, we found that the cell walls were well stained by calcofluor in BY-SUTG cells than in wild type BY-2 cells. These results suggest that NtSUT4 is involved in cell shape via sucrose homeostasis in plant cells.
- Subjects :
- Cell Line
Cell Membrane metabolism
Cell Wall metabolism
Cellulose biosynthesis
Cloning, Molecular
Cytoplasm metabolism
Genetic Complementation Test
Homeostasis
Phylogeny
Plants, Genetically Modified cytology
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Protoplasts cytology
Sequence Analysis, Protein
Nicotiana cytology
Nicotiana genetics
Membrane Transport Proteins metabolism
Plant Proteins metabolism
Sucrose metabolism
Nicotiana metabolism
Vacuoles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0860
- Volume :
- 124
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of plant research
- Publication Type :
- Academic Journal
- Accession number :
- 20941637
- Full Text :
- https://doi.org/10.1007/s10265-010-0377-7