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Interactions of pancreatic secretory trypsin inhibitor in small intestinal juice: its hydrolysis and protection by intraluminal factors.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 1990 Dec 31; Vol. 195 (1-2), pp. 27-39. - Publication Year :
- 1990
-
Abstract
- It has been proposed that modulation of cholecystokinin (CCK) release by proteinases, proteinase inhibitors and protein is mediated by a pancreatic secretory trypsin inhibitor (PSTI), also called monitor peptide, in the rat. When human [125I]-PSTI was incubated with fasting small bowel juice or activated pancreatic juice greater than 88% of tracer eluted from gel chromatography in the characteristic position of hydrolysed PSTI. However, when the small bowel juice had been pre-incubated with soybean trypsin inhibitor 3 g/l, casein 5 g/l or lactalbumin 30 g/l, the hydrolysis of PSTI diminished so that 95%, 32%, and 33% respectively, now eluted in the characteristic position of free (i.e. intact and not bound to an enzyme) PSTI. When [125I]-PSTI was incubated with pure trypsin, chymotrypsin, elastase or enterokinase greater than 95% of tracer eluted in the position of PSTI-enzyme complex. Incubation of PSTI with trypsin plus one other enzyme was required to produce hydrolysis. The degree of protection of PSTI from hydrolysis in duodenal juice produced by these substances correlates with their affects on CCK release. Our findings support the hypothesis that PSTIs mediate the modulation of CCK release by intraluminal proteinases, proteinase inhibitors and proteins.
- Subjects :
- Body Fluids metabolism
Cholecystokinin metabolism
Chromatography
Chymotrypsin metabolism
Enteropeptidase metabolism
Humans
Hydrolysis
Molecular Weight
Pancreatic Elastase metabolism
Pancreatic Juice chemistry
Pancreatic Juice metabolism
Trypsin metabolism
Trypsin Inhibitor, Kazal Pancreatic isolation & purification
Intestine, Small metabolism
Trypsin Inhibitor, Kazal Pancreatic metabolism
Trypsin Inhibitors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0009-8981
- Volume :
- 195
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2093478
- Full Text :
- https://doi.org/10.1016/0009-8981(90)90191-t