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Chromatin and DNA methylation dynamics of Helicobacter pylori-induced COX-2 activation.

Authors :
Pero R
Peluso S
Angrisano T
Tuccillo C
Sacchetti S
Keller S
Tomaiuolo R
Bruni CB
Lembo F
Chiariotti L
Source :
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2011 Feb; Vol. 301 (2), pp. 140-9. Date of Electronic Publication: 2010 Oct 08.
Publication Year :
2011

Abstract

COX-2 expression is altered in gastrointestinal diseases. Helicobacter pylori (Hp) infection may have a critical role in COX-2 disregulation. We undertook this study to investigate possible chromatin and DNA methylation changes occurring early during COX-2 gene activation as a direct consequence of Hp-gastric cells interaction. We show that Hp infection is followed by different expression, chromatin and DNA methylation changes including: (i) biphasic activation of COX-2 gene; (ii) rapid remodulation of HDACs expression and activity, increased acetylation and release of HDAC from COX-2 promoter; (iii) transient gradual increase of H3 acetylation and H3K4 dimethylation and decrease of H3K9 dimethylation; (iv) late and long-lasting increase of H3K27 trimethylation; (v) rapid cyclical DNA methylation/demethylation events at 8 specific CpG sites (-176, -136, +25, +36, +57, +82, +198, +231) surrounding the COX-2 gene transcriptional start site. Our data indicate that specific chromatin and DNA methylation changes occur at COX-2 gene in the first phases of Hp exposure in cultured gastric cells as a primary response to host-parasite interaction.<br /> (Copyright © 2010 Elsevier GmbH. All rights reserved.)

Details

Language :
English
ISSN :
1618-0607
Volume :
301
Issue :
2
Database :
MEDLINE
Journal :
International journal of medical microbiology : IJMM
Publication Type :
Academic Journal
Accession number :
20934379
Full Text :
https://doi.org/10.1016/j.ijmm.2010.06.009