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Chromatin and DNA methylation dynamics of Helicobacter pylori-induced COX-2 activation.
- Source :
-
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2011 Feb; Vol. 301 (2), pp. 140-9. Date of Electronic Publication: 2010 Oct 08. - Publication Year :
- 2011
-
Abstract
- COX-2 expression is altered in gastrointestinal diseases. Helicobacter pylori (Hp) infection may have a critical role in COX-2 disregulation. We undertook this study to investigate possible chromatin and DNA methylation changes occurring early during COX-2 gene activation as a direct consequence of Hp-gastric cells interaction. We show that Hp infection is followed by different expression, chromatin and DNA methylation changes including: (i) biphasic activation of COX-2 gene; (ii) rapid remodulation of HDACs expression and activity, increased acetylation and release of HDAC from COX-2 promoter; (iii) transient gradual increase of H3 acetylation and H3K4 dimethylation and decrease of H3K9 dimethylation; (iv) late and long-lasting increase of H3K27 trimethylation; (v) rapid cyclical DNA methylation/demethylation events at 8 specific CpG sites (-176, -136, +25, +36, +57, +82, +198, +231) surrounding the COX-2 gene transcriptional start site. Our data indicate that specific chromatin and DNA methylation changes occur at COX-2 gene in the first phases of Hp exposure in cultured gastric cells as a primary response to host-parasite interaction.<br /> (Copyright © 2010 Elsevier GmbH. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1618-0607
- Volume :
- 301
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of medical microbiology : IJMM
- Publication Type :
- Academic Journal
- Accession number :
- 20934379
- Full Text :
- https://doi.org/10.1016/j.ijmm.2010.06.009