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Production of polyhydroxyalkanoates (PHAs) with canola oil as carbon source.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2011 Jan 01; Vol. 48 (1), pp. 74-80. Date of Electronic Publication: 2010 Oct 08. - Publication Year :
- 2011
-
Abstract
- Wautersia eutropha was able to synthesize medium chain length polyhydroxyalkanoates (PHAs) when canola oil was used as carbon source. W. eutropha was cultivated using fructose and ammonium sulphate as carbon and nitrogen sources, respectively, for growth and inoculum development. The experiments were done in a laboratory scale bioreactor in three stages. Initially, the biomass was adapted in a batch culture. Secondly, a fed-batch was used to increase the cell dry weight and PHA concentration to 4.36 g L(-1) and 0.36 g L(-1), respectively. Finally, after the addition of canola oil as carbon source a final concentration of 18.27 g L(-1) PHA was obtained after 40 h of fermentation. With canola oil as carbon source, the polymer content of the cell dry matter was 90%. The polymer was purified from dried cells and analyzed by FTIR, NMR and DSC using PHB as reference. The polymer produced by W. eutropha from canola oil had four carbon monomers in the structure of the PHA and identified by 1H and 13C NMR analysis as 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 3-hydroxyoctanoate (3HO), and 3-hydroxydodecanoate (3HDD).<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Biomass
Calorimetry, Differential Scanning
Cupriavidus necator cytology
Cupriavidus necator drug effects
Cupriavidus necator metabolism
Fermentation drug effects
Fructose analysis
Magnetic Resonance Spectroscopy
Microscopy, Fluorescence
Polyhydroxyalkanoates analysis
Quaternary Ammonium Compounds analysis
Rapeseed Oil
Spectroscopy, Fourier Transform Infrared
Transition Temperature drug effects
Carbon pharmacology
Fatty Acids, Monounsaturated pharmacology
Polyhydroxyalkanoates biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 48
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 20933541
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2010.09.016