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[Study on the apoptosis inducing effect of pseudolaric acid B on cervical carcinoma cell line HeLa cells].
- Source :
-
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine [Zhongguo Zhong Xi Yi Jie He Za Zhi] 2010 Jul; Vol. 30 (7), pp. 720-2. - Publication Year :
- 2010
-
Abstract
- Objective: To investigate the biological effect and mechanism of action of pseudolaric acid B (PAB) on the cultured human HeLa cells in vitro.<br />Methods: MTT and flow cytometric assays were used to detect the cells proliferation inhibitory rate and apoptosis of HeLa cell in exposure to PAB; the morphological changes of apoptosis were observed with electron microscope; and the expressions of p53/bcl-2/bax mRNA were detected by RT-PCR.<br />Results: (1) HeLa cell proliferation was inhibited by PAB in a dose-dependent manner, the IC50 being about 10 micromol/L; (2) flow cytometry showed that the distribution of HeLa cell cycle was changed time-dependently by 10 micromol/L PAB-treatment, showing decrease of G0/G1 phase cell percentage and increase of G2/M phase cell percentage; (3) the bax mRNA expression elevated and bcl-2 protein expression decreased markedly after being treated by 10 micromol/L PAB for 12 h, 24 h, and 48 h; while the expression of p53 mRNA could not be detected.<br />Conclusion: PAB can inhibit the proliferation and induce the apoptosis of Hela cells in vitro, and its molecular mechanism may be associated with up-regulating bax mRNA expression and down-regulating bcl-2 mRNA expression.
- Subjects :
- Cell Proliferation drug effects
HeLa Cells
Humans
Proto-Oncogene Proteins c-bcl-2 genetics
Proto-Oncogene Proteins c-bcl-2 metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Tumor Suppressor Protein p53 genetics
Tumor Suppressor Protein p53 metabolism
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis drug effects
Diterpenes pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1003-5370
- Volume :
- 30
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
- Publication Type :
- Academic Journal
- Accession number :
- 20929130