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Molecular and physiological characteristics of a grape yeast strain containing atypical genetic material.
- Source :
-
International journal of food microbiology [Int J Food Microbiol] 2010 Nov 15; Vol. 144 (1), pp. 72-80. Date of Electronic Publication: 2010 Aug 25. - Publication Year :
- 2010
-
Abstract
- The knowledge about wine yeasts remains largely dominated by the extensive studies on Saccharomyces (S.) cerevisiae. Molecular methods, allowing discrimination of both species and strains in winemaking, can profitably be applied for characterization of the microflora occurring in winemaking and for monitoring the fermentation process. Recently, some novel yeast isolates have been described as hybrid between S. cerevisiae and Saccharomyces species, leaving the Saccharomyces strains containing non-Saccharomyces hybrids essentially unexplored. In this study, we have analyzed a yeast strain isolated from "Primitivo" grape (http://www.ispa.cnr.it/index.php?page=collezioni&lang=en accession number 12998) and we found that, in addition to the S. cerevisiae genome, it has acquired genetic material from a non-Saccharomyces species. The study was focused on the analysis of chromosomal and mitochondrial gene sequences (ITS and 26S rRNA, SSU and COXII, ACTIN-1 and TEF), 2D-PAGE mitochondrial proteins, and spore viability. The results allowed us to formulate the hypothesis that in the MSH199 isolate a DNA containing an rDNA sequence from Hanseniaspora vineae, a non-Saccharomyces yeast, was incorporated through homologous recombination in the grape environment where yeast species are propagated. Moreover, physiological characterization showed that the MSH199 isolate possesses high technological quality traits (fermentation performance) and glycerol production, resistance to ethanol, SO₂ and temperature) useful for industrial application.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Carbon Dioxide metabolism
DNA, Fungal genetics
Fermentation
Genome, Fungal genetics
Glycerol metabolism
Hanseniaspora genetics
Hanseniaspora growth & development
Hanseniaspora metabolism
Karyotyping
Mitochondrial Proteins genetics
Saccharomyces cerevisiae growth & development
Spores, Fungal growth & development
Stress, Physiological physiology
Sulfur Dioxide metabolism
Wine microbiology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Vitis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3460
- Volume :
- 144
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 20880601
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2010.08.013