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Three-dimensional reconstruction methods for Caenorhabditis elegans ultrastructure.
- Source :
-
Methods in cell biology [Methods Cell Biol] 2010; Vol. 96, pp. 331-61. - Publication Year :
- 2010
-
Abstract
- The roundworm Caenorhabditis elegans is one of the major model organisms in modern cell and developmental biology. Here, we present methods for the three-dimensional (3D) reconstruction of the worm ultrastructure. We describe the use of (1) serial-section analysis, (2) electron tomography, and (3) serial block face imaging by scanning electron microscopy (SEM). Sample preparation for high-pressure freezing/freeze substitution (HPF/FS) has been extensively covered in a previous volume of this "Methods in Cell Biology" series and will only be described briefly. We will discuss these 3D methods in light of recent research activities related to worm and early embryo biology.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Electron Microscope Tomography instrumentation
Freeze Substitution instrumentation
Freeze Substitution methods
Image Processing, Computer-Assisted instrumentation
Image Processing, Computer-Assisted methods
Imaging, Three-Dimensional instrumentation
Microscopy instrumentation
Microscopy, Electron, Scanning instrumentation
Tissue Fixation methods
Caenorhabditis elegans ultrastructure
Electron Microscope Tomography methods
Imaging, Three-Dimensional methods
Microscopy methods
Microscopy, Electron, Scanning methods
Subjects
Details
- Language :
- English
- ISSN :
- 0091-679X
- Volume :
- 96
- Database :
- MEDLINE
- Journal :
- Methods in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 20869530
- Full Text :
- https://doi.org/10.1016/S0091-679X(10)96015-9