Effect of double-stranded DNA on maturation of dendritic cells in vitro.

Authors :: Alyamkina EA
Leplina OY
Sakhno LV
Chernykh ER
Ostanin AA
Efremov YR
Shilov AG
Proskurina AS
Orishchenko KE
Dolgova EV
Rogachev VA
Nikolin VP
Popova NA
Zagrebelniy SN
Bogachev SS
Shurdov MA
Source :: Cellular immunology [Cell Immunol] 2010; Vol. 266 (1), pp. 46-51. Date of Electronic Publication: 2010 Sep 21.
Publication Year :: 2010
Abstract: A preparation of human genomic fragmented double-stranded DNA (dsDNA) was used as maturation stimulus in cultures of human dendritic cells (DCs) generated in compliance with the interferon protocol. Culturing of the DCs in medium with 5μg/ml of the DNA preparation was associated with a decrease in the relative proportion of CD14 + cells and an increase in that of CD83 + cells. These changes are markers of DC maturation. The efficiency with which the DNA preparation was able to elicit DC maturation was commensurate with that of lypopolysaccharide from bacterial cell, the standard inducer of DC maturation. Generated ex vivo, matured in the presence of the human DNA preparation, pulsed with tumor antigens mouse DCs were used as a vaccine in biological tests for its antitumor activity. The experimental results demonstrate that reinfusion of mature pulsed with tumor antigens DCs cause a statistically significant suppression of tumor graft growth.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)