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The expression and function of β-1,4-galactosyltransferase-I in dendritic cells.
- Source :
-
Cellular immunology [Cell Immunol] 2010; Vol. 266 (1), pp. 32-9. Date of Electronic Publication: 2010 Aug 31. - Publication Year :
- 2010
-
Abstract
- β-1,4-Galactosyltransferase-I (GalTI) is unusual among the galactosyltransferase family, which has two isoforms that differ only in the length of their cytoplasmic domains [1]. In this study, we found that both the long and short isoforms of GalTI were expressed in human monocyte-derived dendritic cells (MoDCs), and localized in the cytoplasm near nucleus and cytomembrane. The expression level of GalTI and cellular adhesion ability was increased when DCs continued to mature. We also demonstrated that the cellular adhesion ability of DCs was inhibited by α-lactalbumin (α-LA) via interference with cell surface GalTI function, suggesting that the adhesion ability was positively correlated with the expression of cell surface long GalTI. α-LA also could inhibit DC-T clustering and CD4(+) T cell proliferation. Collectively, the data suggests that GalTI might act as a key adhesion molecular participating in T cells-DCs contacts.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Antigens, Differentiation metabolism
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes immunology
Cell Adhesion drug effects
Cell Differentiation drug effects
Cell Membrane enzymology
Cell Proliferation drug effects
Cytoplasm enzymology
Dendritic Cells cytology
Dendritic Cells immunology
Galactosyltransferases antagonists & inhibitors
Galactosyltransferases genetics
Gene Expression genetics
Humans
Immunological Synapses enzymology
Isoenzymes genetics
Lactalbumin pharmacology
Laminin metabolism
Lymphocyte Activation drug effects
Lymphocyte Activation immunology
Monocytes cytology
Monocytes drug effects
Cell Adhesion immunology
Dendritic Cells enzymology
Galactosyltransferases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2163
- Volume :
- 266
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20851383
- Full Text :
- https://doi.org/10.1016/j.cellimm.2010.08.008