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Microarray-based detection of virulence genes in verotoxigenic Escherichia coli O157:H7 strains from Swedish cattle.
- Source :
-
Epidemiology and infection [Epidemiol Infect] 2011 Jul; Vol. 139 (7), pp. 1088-96. Date of Electronic Publication: 2010 Sep 15. - Publication Year :
- 2011
-
Abstract
- Verotoxigenic Escherichia coli (VTEC) serotype O157:H7 strains from a Swedish cattle prevalence study (n=32), and livestock-derived strains linked to human disease (n=13), were characterized by microarray and PCR detection of virulence genes. The overall aim of the study was to investigate the distribution of known virulence determinants and determine which genes are linked to increased pathogenicity in humans. A core set of 18 genes or gene variants were found in all strains, while seven genes were variably present. This suggests that the majority of VTEC O157:H7 found in Swedish cattle carry a broad repertoire of virulence genes and should be considered potentially harmful to humans. A single virulence gene type was significantly associated with strains linked to human disease cases (P=0.012), but no genetic trait to explain the increased virulence of this genotype could be found.
- Subjects :
- Animals
Cattle
Cattle Diseases microbiology
Electrophoresis, Gel, Pulsed-Field
Escherichia coli Infections microbiology
Escherichia coli O157 pathogenicity
Genes, Bacterial genetics
Humans
Oligonucleotide Array Sequence Analysis methods
Polymerase Chain Reaction
Prevalence
Sweden epidemiology
Virulence genetics
Cattle Diseases epidemiology
Escherichia coli Infections veterinary
Escherichia coli O157 genetics
Oligonucleotide Array Sequence Analysis veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1469-4409
- Volume :
- 139
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Epidemiology and infection
- Publication Type :
- Academic Journal
- Accession number :
- 20843386
- Full Text :
- https://doi.org/10.1017/S095026881000213X