Back to Search Start Over

Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus.

Authors :
Campos VF
Collares T
Deschamps JC
Seixas FK
Dellagostin OA
Lanes CF
Sandrini J
Marins LF
Okamoto M
Sampaio LA
Robaldo RB
Source :
Journal of biosciences [J Biosci] 2010 Sep; Vol. 35 (3), pp. 405-13.
Publication Year :
2010

Abstract

Neuropeptide Y (NPY) is one of the most potent stimulants of food intake in vertebrates, mammals and fish. However, the present knowledge about feeding behaviour in fish is still limited and based on studies in a few species. The Brazilian flounder Paralichthys orbignyanus is being considered for aquaculture, and it is important to understand the mechanisms regulating feeding in order to improve its performance in captivity. The objectives of this study were to clone NPY cDNA, evaluate the mRNA levels in different tissues of flounder, and also evaluate brain NPY expression to associate food intake with NPY expression levels. A 597 bp NPY cDNA was cloned from Brazilian flounder brain. NPY expression was detected in all the peripheral tissues analysed. No significant differences were observed in brain NPY gene expression over 24 h after food intake at a temperature of 15 +or- 3 degrees C. No correlation was observed among plasma glucose, total protein, cholesterol, triglycerides and NPY expression levels during this 24 h period. On the other hand, mRNA levels were increased after two weeks of fasting at elevated temperatures. Our results suggest that NPY mRNA levels in Brazilian flounder are affected by temperature.

Details

Language :
English
ISSN :
0973-7138
Volume :
35
Issue :
3
Database :
MEDLINE
Journal :
Journal of biosciences
Publication Type :
Academic Journal
Accession number :
20826950
Full Text :
https://doi.org/10.1007/s12038-010-0046-y