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Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells.
- Source :
-
Journal of Zhejiang University. Science. B [J Zhejiang Univ Sci B] 2010 Sep; Vol. 11 (9), pp. 719-27. - Publication Year :
- 2010
-
Abstract
- Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated 'gene silencing' of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.
- Subjects :
- Animals
Cell Cycle physiology
Cell Cycle Proteins chemistry
Cell Cycle Proteins genetics
Estradiol blood
Female
Polymerase Chain Reaction
Progesterone blood
RNA chemistry
RNA Interference physiology
Smad4 Protein genetics
Apoptosis physiology
Bone Morphogenetic Proteins physiology
Granulosa Cells physiology
RNA genetics
Signal Transduction physiology
Smad4 Protein physiology
Swine physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1862-1783
- Volume :
- 11
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of Zhejiang University. Science. B
- Publication Type :
- Academic Journal
- Accession number :
- 20803776
- Full Text :
- https://doi.org/10.1631/jzus.B1000079