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Determination of aniracetam and its main metabolite, N-anisoyl-GABA, in human plasma by high-performance liquid chromatography.
- Source :
-
Journal of chromatography [J Chromatogr] 1990 Sep 14; Vol. 530 (2), pp. 397-406. - Publication Year :
- 1990
-
Abstract
- Two different reversed-phase high-performance liquid chromatographic methods for the determination of aniracetam (I) and its metabolite N-anisoyl-GABA (II) in human plasma are described. The procedure for I involves direct injection of plasma samples spiked with the internal standard on a clean-up column followed by reversed-phase chromatography on a C18 column. The limit of quantification was 5 ng/ml, using a 200-microliters specimen of plasma. The mean inter-assay precision of the method up to 800 ng/ml was 3%. The procedure for II involved liquid-liquid extraction of II and the internal standard from plasma with ethyl acetate, and reversed-phase chromatography on a C18 column. The limit of quantification was 50 ng/ml using a 0.5-ml plasma specimen. The mean inter-assay precision up to 50 micrograms/ml was 6%. The applicability and accuracy of the methods were demonstrated by the analysis of over 1000 plasma samples from two bioavailability studies in healthy volunteers.
Details
- Language :
- English
- Volume :
- 530
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 2079514
- Full Text :
- https://doi.org/10.1016/s0378-4347(00)82342-7