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Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ.
- Source :
-
The Journal of cell biology [J Cell Biol] 2010 Aug 23; Vol. 190 (4), pp. 613-21. - Publication Year :
- 2010
-
Abstract
- Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a "hibernation state." Several proteins have been identified that are associated with 100S ribosomes but their spatial organization has hitherto not been characterized. We have used cryoelectron tomography to reveal the three-dimensional configuration of 100S ribosomes isolated from starved Escherichia coli cells and we have described their mode of interaction. In situ studies with intact E. coli cells allowed us to demonstrate that 100S ribosomes do exist in vivo and represent an easily reversible state of quiescence; they readily vanish when the growth medium is replenished.
- Subjects :
- Escherichia coli cytology
Escherichia coli metabolism
Escherichia coli Proteins analysis
Models, Molecular
Molecular Sequence Data
Ribosomal Proteins analysis
Ribosomes metabolism
Cryoelectron Microscopy methods
Nucleic Acid Conformation
Protein Conformation
Ribosomes chemistry
Tomography methods
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8140
- Volume :
- 190
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 20733057
- Full Text :
- https://doi.org/10.1083/jcb.201005007