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Development of a sphingosylphosphorylcholine detection system using RNA aptamers.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2010 Aug 20; Vol. 15 (8), pp. 5742-55. Date of Electronic Publication: 2010 Aug 20. - Publication Year :
- 2010
-
Abstract
- Sphingosylphosphorylcholine (SPC) is a lysosphingolipid that exerts multiple functions, including acting as a spasmogen, as a mitogenic factor for various types of cells, and sometimes as an inflammatory mediator. Currently, liquid chromatography/tandem mass spectrometry (LC/MS/MS) is used for the quantitation of SPC. However, because of the complicated procedures required it may not be cost effective, hampering its regular usage in a routine practical SPC monitoring. In this report, we have generated RNA aptamers that bind to SPC with high affinity using an in vitro selection procedure and developed an enzyme-linked aptamer assay system using the minimized SPC aptamer that can successfully distinguish SPC from the structurally related sphingosine 1-phosphate (S1P). This is the first case of the Systematic Evolution of Ligands by EXponential enrichment (SELEX) process being performed with a lysosphingolipid. The SPC aptamers would be valuable tools for the development of aptamer-based medical diagnosis and for elucidating the biological role of SPC.
- Subjects :
- Aptamers, Nucleotide genetics
Base Sequence
Biological Assay
Kinetics
Molecular Sequence Data
Nucleic Acid Conformation
Phosphorylcholine analysis
Phosphorylcholine chemistry
Reproducibility of Results
Sphingosine analysis
Sphingosine chemistry
Surface Plasmon Resonance
Aptamers, Nucleotide chemistry
Phosphorylcholine analogs & derivatives
SELEX Aptamer Technique methods
Sphingosine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 15
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 20729797
- Full Text :
- https://doi.org/10.3390/molecules15085742