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Epidermal growth factor regulates PAI-1 expression via activation of the transcription factor Elk-1.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2010 Sep; Vol. 1799 (9), pp. 616-21. Date of Electronic Publication: 2010 Aug 19. - Publication Year :
- 2010
-
Abstract
- PAI-1 (plasminogen activator inhibitor-1) in breast cancer cells is involved in tumour development and metastasis of breast cancer cells. PAI-1 function and the regulation of its expression have been precisely investigated. Here we report that EGF, which promotes breast cancer tumour growth and survival, rapidly induces PAI-1 expression in the breast adenocarcinoma cell line MCF-7 through the activation of the transcription factor Elk-1. We have found that the PAI-1 promoter fragment (-140 to +173) containing the Ets consensus binding site is activated by Elk-1. Chromatin immunoprecipitation analysis confirms in vivo binding of Elk-1 to the PAI-1 promoter and demonstrates that Elk-1 phosphorylation on the Ets binding site is EGF-dependent.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Adenocarcinoma genetics
Adenocarcinoma metabolism
Adenocarcinoma pathology
Binding Sites drug effects
Breast Neoplasms genetics
Breast Neoplasms metabolism
Breast Neoplasms pathology
Epidermal Growth Factor pharmacology
Female
Gene Expression Regulation, Neoplastic drug effects
Humans
Phosphorylation drug effects
Plasminogen Activator Inhibitor 1 metabolism
Promoter Regions, Genetic drug effects
Protein Binding drug effects
Protein Kinases metabolism
Proto-Oncogene Protein c-ets-1 metabolism
Transcriptional Activation drug effects
Transfection
Tumor Cells, Cultured
ets-Domain Protein Elk-1 physiology
Epidermal Growth Factor physiology
Plasminogen Activator Inhibitor 1 genetics
ets-Domain Protein Elk-1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1799
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 20727996
- Full Text :
- https://doi.org/10.1016/j.bbagrm.2010.08.004