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Structural and functional profile of the carbohydrate esterase gene complement in Phytophthora infestans.
- Source :
-
Current genetics [Curr Genet] 2010 Dec; Vol. 56 (6), pp. 495-506. Date of Electronic Publication: 2010 Aug 20. - Publication Year :
- 2010
-
Abstract
- The plant cell cuticle is the first obstacle for penetration of the host by plant pathogens. To breach this barrier, most pathogenic fungi employ a complex assortment of cell wall-degrading enzymes including carbohydrate esterases, glycoside hydrolases, and polysaccharide lyases. We characterized the full complement of carbohydrate esterase-coding genes in three Phytophthora species and analyzed the expression of cutinase in vitro and in planta; we also determined the cutinase allele distribution in multiple isolates of P. infestans. Our investigations revealed that there are 49, 21, and 37 esterase homologs in the P. infestans, P. ramorum, and P. sojae genomes, respectively, with a considerable number predicted to be extracellular. Four cutinase gene copies were found in both the P. infestans and P. ramorum genomes, while 16 copies were found in P. sojae. Transcriptional analyses of cutinase in P. infestans revealed that its expression level during infection is significantly upregulated at all time points compared to that of the same gene in mycelium grown in vitro. Expression achieves maximum values at 15 hpi, declining at subsequent time points. These results may suggest, therefore, that cutinase most likely plays a role in P. infestans pathogenicity.
- Subjects :
- Carboxylic Ester Hydrolases genetics
Carboxylic Ester Hydrolases metabolism
Carboxylic Ester Hydrolases physiology
Chromosome Mapping
Cloning, Molecular
DNA, Complementary isolation & purification
DNA, Complementary metabolism
DNA, Complementary physiology
Esterases metabolism
Esterases physiology
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Models, Biological
Phylogeny
Phytophthora infestans enzymology
Phytophthora infestans metabolism
Phytophthora infestans pathogenicity
Sequence Homology
Carbohydrate Metabolism genetics
DNA, Complementary analysis
Esterases genetics
Phytophthora infestans genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0983
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 20725833
- Full Text :
- https://doi.org/10.1007/s00294-010-0317-z