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Osteogenic differentiation of intact human amniotic membrane.
- Source :
-
Biomaterials [Biomaterials] 2010 Nov; Vol. 31 (33), pp. 8659-65. Date of Electronic Publication: 2010 Aug 17. - Publication Year :
- 2010
-
Abstract
- Tissue engineering strategies usually require cell isolation and combination with a suitable biomaterial. Human amniotic membrane (AM) represents a natural two-layered sheet comprising cells with proven stem cell characteristics. In our approach, we evaluated the differentiation potential of AM in toto with its sessile stem cells as alternative to conventional approaches requiring cell isolation and combination with biomaterials. For this, AM-biopsies were differentiated in vitro using two osteogenic media compared with control medium (CM) for 28 days. Mineralization and osteocalcin expression was demonstrated by (immuno)histochemistry. Alkaline phosphatase (AP) activity, calcium contents and mRNA expression of RUNX2, AP, osteopontin, osteocalcin, BMP-2 (bone morphogenetic protein), and BMP-4 were quantified and AM viability was evaluated. Under osteogenic conditions, AM-biopsies mineralized successfully and by day 28 the majority of cells expressed osteocalcin. This was confirmed by a significant rise in calcium contents (up to 27.4 ± 6.8 mg/dl d28), increased AP activity, and induction of RUNX2, AP, BMP-2 and BMP-4 mRNA expression. Relatively high levels of viability were retained, especially in osteogenic media (up to 78.3 ± 19.0% d14; 62.9 ± 22.3% d28) compared to CM (42.2 ± 15.2% d14; 35.1 ± 8.6% d28). By this strategy, stem cells within human AM can successfully be driven along the osteogenic pathways while residing within their natural environment.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Alkaline Phosphatase metabolism
Amnion drug effects
Amnion enzymology
Biomarkers metabolism
Calcium metabolism
Cell Survival drug effects
Cells, Cultured
Culture Media pharmacology
Gene Expression Regulation drug effects
Humans
Intracellular Space drug effects
Intracellular Space enzymology
Reverse Transcriptase Polymerase Chain Reaction
Amnion cytology
Cell Differentiation drug effects
Osteogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 31
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 20719379
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.07.090