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Decay accelerating factor (CD55)-mediated attenuation of complement: therapeutic implications for age-related macular degeneration.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2010 Dec; Vol. 51 (12), pp. 6776-83. Date of Electronic Publication: 2010 Aug 04. - Publication Year :
- 2010
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Abstract
- Purpose: Sequence variations in complement proteins are associated with age-related macular degeneration (AMD). The terminal pathway of complement results in the formation of the membrane attack complex (MAC) on the cell surface, resulting in their lysis. MAC has been documented on the retinal pigment epithelium (RPE), choroidal blood vessels, and drusen of AMD eyes. Here the investigators test the hypothesis that increasing the expression of decay accelerating factor (CD55) on RPE cells may result in reduced MAC-mediated damage.<br />Methods: The investigators constructed a recombinant adenovirus expressing human CD55 (AdCAGCD55). Mouse hepatocytes were infected with AdCAGCD55 or negative controls and subsequently incubated with normal human serum (NHS). Cell lysis and MAC formation were measured by FACS and immunocytochemistry, respectively. Adult mice were injected in the subretinal space with either AdCAGCD55 or controls; after 1 week of CD55 transgene expression, the eyecups were excised, challenged with NHS, and quantified for human MAC formation.<br />Results: Control-infected or uninfected mouse hepatocytes lyse at a rate of 93% and 94%, respectively. AdCAGCD55- infected mouse hepatocytes lyse at a rate of 29%. Lysis was confirmed to occur in the presence of MAC, which was reduced by 67% when cells were infected by AdCAGCD55. Mice injected in the subretinal space with AdCAGCD55 exhibited a 55.7% reduction in MAC formation on the RPE relative to controls.<br />Conclusions: Adenovirus-mediated delivery of hCD55 to murine RPE confers protection against human complement. The investigators propose that the expression of hCD55 on RPE cells warrants investigation as a potential therapy for AMD.
- Subjects :
- Adenoviridae genetics
Animals
Blotting, Western
CD55 Antigens genetics
Cell Line
Flow Cytometry
Gene Expression physiology
Genetic Vectors
Hemolysis physiology
Hepatocytes metabolism
Hepatocytes virology
Humans
Immunohistochemistry
Macular Degeneration metabolism
Mice
Mice, Inbred C57BL
Transgenes
CD55 Antigens therapeutic use
Complement Membrane Attack Complex metabolism
Complement System Proteins physiology
Genetic Therapy
Macular Degeneration therapy
Retinal Pigment Epithelium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 51
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 20688727
- Full Text :
- https://doi.org/10.1167/iovs.10-5887