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Diagnostic accuracy and feasibility of serological tests on filter paper samples for outbreak detection of T.b. gambiense human African trypanosomiasis.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2010 Aug; Vol. 83 (2), pp. 374-9. - Publication Year :
- 2010
-
Abstract
- Control of human African trypanosomiasis (HAT) in the Democratic Republic of Congo is based on mass population screening by mobile teams; a costly and labor-intensive approach. We hypothesized that blood samples collected on filter paper by village health workers and processed in a central laboratory might be a cost-effective alternative. We estimated sensitivity and specificity of micro-card agglutination test for trypanosomiasis (micro-CATT) and enzyme-linked immunosorbent assay (ELISA)/T.b. gambiense on filter paper samples compared with parasitology-based case classification and used the results in a Monte Carlo simulation of a lot quality assurance sampling (LQAS) approach. Micro-CATT and ELISA/T.b. gambiense showed acceptable sensitivity (92.7% [95% CI 87.4-98.0%] and 82.2% [95% CI 75.3-90.4%]) and very high specificity (99.4% [95% CI 99.0-99.9%] and 99.8% [95% CI 99.5-100%]), respectively. Conditional on high sample size per lot (> or = 60%), both tests could reliably distinguish a 2% from a zero prevalence at village level. Alternatively, these tests could be used to identify individual HAT suspects for subsequent confirmation.
- Subjects :
- Antibodies, Protozoan blood
Blood Specimen Collection instrumentation
Blood Specimen Collection methods
Democratic Republic of the Congo epidemiology
Filtration instrumentation
Humans
Latex Fixation Tests standards
Monte Carlo Method
Paper
ROC Curve
Sensitivity and Specificity
Specimen Handling
Trypanosomiasis, African epidemiology
Agglutination Tests standards
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay standards
Trypanosoma brucei gambiense immunology
Trypanosomiasis, African diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1476-1645
- Volume :
- 83
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 20682885
- Full Text :
- https://doi.org/10.4269/ajtmh.2010.09-0735